Shifting from CO2-saturated to CO2-limited conditions decreased total oxidizable P700 to approximately 10% in the flv knockout mutant of S. 7002 but not in those of S. 6803 or S. 7942. The key difference between photosystem 1 and photosystem 2 is that the photosystem 1 has a reaction centre composing of chlorophyll a molecule of P700 that absorbs light at a wavelength of 700 nm. For S. 6803, we used a mutant deficient in the expression of all four flv genes (Δflv1/3/4), since the wild type of this species possesses FLV2/4 (Table I; Supplemental Fig. Note the C 2-symmetric arrangement of cofactors P700, A 0, A 1, and F X, all of which are bound by two core, membrane-spanning subunits PsaA and PsaB; as well as the symmetry-breaking arrangement of cofactors F A and F B, bound by stromal subunit PsaC. Supplemental Figure S1. Oxidation of P700 in Photosystem I Is Essential for the Growth of Cyanobacteria, Flavodiiron proteins Flv1 and Flv3 enable cyanobacterial growth and photosynthesis under fluctuating light, Simple conditions for growth of unicellular blue-green algae on plates1, 2, Oxygen consumption: photorespiration and chlororespiration, Flavodiiron protein Flv2/Flv4-related photoprotective mechanism dissipates excitation pressure of PSII in cooperation with phycobilisomes in cyanobacteria, Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation, Differential roles of carotenes and xanthophylls in photosystem I photoprotection, The antisense RNA As1_flv4 in the cyanobacterium, CyanoBase and RhizoBase: databases of manually curated annotations for cyanobacterial and rhizobial genomes, The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence, Photochemical activities of a particle fraction P 1 obtained from the green alga Chlorella fusca, Fractionation of the three stable oxygen isotopes by oxygen-producing and oxygen-consuming reactions in photosynthetic organisms, Genes encoding A-type flavoproteins are essential for photoreduction of O, Regulation, inhibition and protection of photosystem I, An improved method, using saturating light pulses, for the determination of photosystem I quantum yield via P700, Saturation pulse method for assessment of energy conversion in PSI, Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature, Range of photosynthetic control of postillumination P700, Thylakoid terminal oxidases are essential for the cyanobacterium, Acclimation of the global transcriptome of the cyanobacterium, Enhancement of cyclic electron flow around PSI at high light and its contribution to the induction of non-photochemical quenching of Chl fluorescence in intact leaves of tobacco plants, Two types of functionally distinct NAD(P)H dehydrogenases in, Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer, Repetitive short-pulse light mainly inactivates photosystem I in sunflower leaves, Reduction-induced suppression of electron flow (RISE) in the photosynthetic electron transport system of, Diversity in photosynthetic electron transport under [CO, Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in, FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent alternative electron flow in, PROTON GRADIENT REGULATION5 is essential for proper acclimation of, Superoxide and singlet oxygen produced within the thylakoid membranes both cause photosystem I photoinhibition, A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K, Kinetic properties and physiological role of the plastoquinone terminal oxidase (PTOX) in a vascular plant, The use of chlorophyll fluorescence nomenclature in plant stress physiology, Module fusion in an A-type flavoprotein from the cyanobacterium, PsaE is required for in vivo cyclic electron flow around photosystem I in the cyanobacterium, Flavodiiron proteins in oxygenic photosynthetic organisms: photoprotection of photosystem II by Flv2 and Flv4 in. The stored energy in the proton gradient is used to produce ATP which is used later in the Calvin-Benson Cycle. The photoinhibition of photosystem I (PSI) is lethal to oxygenic phototrophs. Das Photosystem I (PS I) ist ein Proteinkomplex aus mehreren Membranproteinen, der im Zuge der Photosynthese eine Oxidation von Plastocyanin und die Reduktion von Ferredoxin durch absorbierte Photonen katalysiert. These findings concur with those of previous studies showing that FLV1/3-mediated AEF can oxidize P700 (Helman et al., 2003; Allahverdiyeva et al., 2013; Hayashi et al., 2014). 2, A and B). Recently, it was suggested that 1O2 triggers PSI photoinhibition (Cazzaniga et al., 2012, 2016; Takagi et al., 2016). Several molecular mechanisms are proposed for P700 oxidation wherein the PSI acceptor does not limit the PET reaction. Das Photosystem I enthält insgesamt ca. The mutant of S. 7942 also had a higher Y(ND) than did the S. 7942 wild type under CO2 limitation (Supplemental Fig. Reduced activities of PSI and photosynthesis in S. 7002 wild type (WT) and Δflv1/3 after 2-h exposures to CO2 limitation during illumination (290 µmol photons m−2 s−1). ); 110 Carotinoide. Reaction mixtures contained the cells (10 µg Chl mL−1). 3B). Photosystem II uses a form of chlorophyll a known as P680. of multiple genes (paralogs).
Absorbance changes were monitored as a function of time with 1.5 ps time resolution. Photosystem 1: The photocenter of the photosystem 1 is P700. For Chl measurements, cells from 0.1- to 1-mL cultures were centrifugally harvested and resuspended by vortexing in 1 mL of 100% (v/v) methanol. S1; Eisenhut et al., 2012). This response was not observed in either S. 6803 (Zhang et al., 2009) or S. 7942 (Shaku et al., 2015). Black and red symbols represent the wild type and Δflv1/3, respectively. and by the Core Research for Evolutional Science and Technology of the Japan Science and Technology Agency (grant no. In S. 6803, FLV2/4 may receive electrons from the acceptor side of PSI. S5 and S7). PCC 7002 (S. 7002), however, is linked directly to reduced growth and PSI photodamage under CO2-limiting conditions. It absorbs maximum light of 700nm. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)),This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. Cyclic electron flow around PSI may contribute to surplus Y(I) (see “Discussion”). The nature of the metal is indicated in the 'Description' field.
, Iron-sulfur (4Fe-4S); shared with dimeric partner, Magnesium (chlorophyll-a' A1 axial ligand; P700 special pair), Magnesium (chlorophyll-a A3 axial ligand),This subsection of the Function section describes the interaction between a single amino acid and another chemical entity. For the simultaneous measurements of Y(II), Y(I), Y(ND), and Y(NA), a 300-ms saturation pulse (10,000 µmol photons m−2 s−1) was supplied every 10 min. photosystem I. P700, … Supplemental Figure S6.
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